Figure 2.

Diagram summarizes the principles of fast coamplification at lower denaturation temperature–droplet digital PCR (fast-COLD-ddPCR). Both mutant and wild-type alleles are compartmentalized into thousands of droplets before the thermal cycling step. In conventional ddPCR, both wild-type and mutant amplicons coamplify in different compartments. If fast-COLD-ddPCR is implemented during the thermal cycling inhibition of the wild-type amplicon, preferential amplification of mutation-containing droplets occurs. amp., amplification; mut, mutant; WT, wild type.