Figure 4.
Application of coamplification at lower denaturation temperature–droplet digital PCR (COLD-ddPCR) FAM/HEX ratio method for detection of mutation p.T790M in cell-free circulating DNA samples. Serial dilution samples were also tested in parallel to enable assessment of mutational abundances. Numbers over the histograms indicate mutation abundances derived via independent quantification performed using allele-specific ddPCR for p.T790M as described.24 The error bars represent variations among three independent detections. WT, wild type.