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. 2015 May;17(3):284–292. doi: 10.1016/j.jmoldx.2014.12.003

Figure 6.

Figure 6

Detection of mutations in solid tumor and cell-free circulating DNA (cfDNA) samples by the coamplification at lower denaturation temperature–droplet digital PCR (COLD-ddPCR) FAM/HEX ratio method in TP53 exon 8. A: FAM/HEX ratio value for clinical sample CT20, harboring missense mutation p.R273H (overlapping the HEX-labeled probe). B: FAM/HEX ratio for a cfDNA sample, FC39-4 with the missense mutation p.R283C (overlapping the FAM-labeled probe). C: Two-dimensional plots for CT20 and FC39-4 samples obtained following COLD-ddPCR. The error bars represent variations among three independent detections. Ch1/2, channel 1/2.