Figure 5.

α6 integrin is required for rotational and collective motion. (A) Rotational motion of a two-cell colony of human epidermal keratinocytes and distribution of α6 integrin. Serial images of a two-cell stage colony were collected for 60 min, and then the colony was immediately fixed and stained with an antibody against α6 integrin (17D11). Bars, 20 µm. (B) Distribution of rotational speed of two-cell colonies of keratinocytes transduced with control and ITGA6 shRNA. Rotation score is described in Fig. 1. The data shown (n = 35 and 36 colonies of control shRNA and ITGA6 shRNA-transfected keratinocytes, respectively) were obtained from triplicate experiments. (C) Mixture of ITGA6 shRNA-transduced and nontransduced keratinocytes in a large progressively growing colony. Bar, 50 µm. (D) Effect of α6 integrin knockdown on keratinocyte locomotion in the colony. The locomotion speed of cells was measured after transduction of control and ITGA6 shRNA. shRNA-transduced cells were identified as GFP-positive (GFP+) cells. The values were obtained from analyzing five colonies in each condition. P-values were calculated by Student’s t test.