Figure 2. Cancer-derived cell lines also exhibit a lineage-related transductional bias.

(a) Transductional efficiencies exhibited by 10 breast cancer cell lines, grouped by their mRNA expression profiles. Cell lines were inoculated in parallel with the identical twofold serial dilution series of pLenti6/CMV-ZsGreen lentivirus (inoculated in DMEM/10% FBS, 6 μg ml⁻¹ polybrene) and analysed by FACS. (b) Fluorescent and phase-contrast image overlays of three representative cell lines, MDA-MB-468, MDA-MB-231 and MCF-7, inoculated with pLenti6/CMV-H2B-GFP lentivirus. (c) Flow cytometry analysis of MCF-7 cells inoculated with a twofold dilution series of pLenti6/CMV-H2B-GFP lentivirus (‘Parental,' blue trace). Virally resistant cells (GFP negative) were sorted by FACS from the culture inoculated with the highest dose of virus. These sorted ‘resistant' cells (black trace) were reinoculated with virus and analysed under conditions identical to the previous infection.