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. 2015 Apr 21;6:6899. doi: 10.1038/ncomms7899

Figure 7. Structural comparison of the STIM CAD domains in the presence or absence of Orai1 C-terminal peptide domain.

Figure 7

(a) Homology models of the STIM1, STIM2.2 and STIM2.1 CAD domains. The homology models were built according to the template human STIM1 CAD domain (PDB ID: 3TEQ). The inserted segment in the STIM2.1 model is coloured in pink. (b) Docking the C-terminal helix of Orai1 on STIM dimers. STIM CAD domains are coloured in grey. The conformations of the C-terminal helix of Orai1 were predicted by the docking packages DOT2 (cyan), FRODOCK (magenta) and ZDOCK (yellow). Motif 1: upper part of CAD domain that is supposed to be close to or in contact with the membrane containing ORAI channel proteins. Motif 2: region near the crossing of STIM CAD dimerization domain. Motif 3: the lateral face of STIM CAD near the dimerization domain. (c) Electrostatic potential distribution on the solvent-accessible surfaces of the STIM CAD domains. The unit of electrostatic potential used is kT e−1. Dimerization domain (Dd).