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. 2015 Apr 21;6:6975. doi: 10.1038/ncomms7975

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(a) The Sho1 deletion constructs used in c. TM, transmembrane domains. (b) Schematic model of Sho1. Numbers are amino-acid positions. L, Loop region. (c,d) Sho1–Sho1-binding assays. p424GAL1-GST-Sho1 (or its deletion derivative as indicated) and p416GAL1-Sho1-GFP were co-expressed in KT079 (sho1Δ) cells. GST-Sho1 was affinity purified using glutathione beads, and co-precipitated Sho1-GFP was detected by immunoblotting. (d) 1 M NaCl was added as indicated for 5 min before preparation of cell lysates.