Table 1.

Programme for qRT-PCR validation of differentially expressed genes of p. glaucum

PCR stages	Sub stage	Temperature	Time	Cycles
Pre incubation		95 °C	3 min	1
Amplification	Denaturation	95 °C	10 s	30
	Annealing	60 °C	20 s
	Extension	72 °C	10 s
Mating curve		95 °C	5 s	1
		60 °C	1 min
		97 °C	Continuous
		40 °C	Hold