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SDS–PAGE of the urine samples from rats treated with different diuretics Urine protein samples were separated by SDS–PAGE and stained using Commassie brilliant blue for the hydrochlorothiazide group (H, A), the furosemide group (F, B) and the spirolactone group (S, C). M, markers; B, normal rat urine samples; A1, A3 and A5, urine samples obtained 1, 3, 5 days after the diuretics were administered.
