Table 1.
Strain | No. of raw reads | No. of reads after rRNA removal | No. of mapped reads | No. of uniquely-mapped reads |
No. of TARs |
|
---|---|---|---|---|---|---|
+ strand | − strand | |||||
05ZYH33 | 50,554,946 | 50,553,160 (99.999%) | 47,527,320 (94.01%) | 39,020,066 (82.1%) | 1285 | 1216 |
98HAH33 | 33,429,474 | 33,406,466 (99.93%) | 31,581,564 (94.54%) | 27,182,870 (86.07%) | 1332 | 1211 |
P1/7 | 47,162,906 | 47,139,616 (99.95%) | 44,877,096 (95.2%) | 40,313,416 (92.43%) | 1222 | 1216 |
Note: Different coverage thresholds were used for identification of TARs (>70 bp in length) in the three strains, which are 9 × for 05ZYH33, 7 × for 98HAH33 and 16 × for P1/7, respectively. All the percentages in the parenthesis beside the absolute read No. were obtained by dividing the target read number by the one listed in the preceding column on its left. For example, percentage of “No. of reads after rRNA removal” = “No. of reads after rRNA removal” (50,553,160)/“No. of raw reads” (50,554,946) = 99.99%. TAR, transcriptionally-active region.