Effects of alendronate on the apparent characteristics of isoprene synthase, νm,iso,app and Km,iso,app, in young 6-7-day-old (a) and mature 20-day-old leaves (b) of aspen treated for various times with alendronate. The isoprene synthase characteristics were derived from Hanes-Wolff plots as in Fig. S1.2 (Supplemental Material S1). If isoprene synthesis was the only process consuming DMADP as is expected in alendronate-inhibited leaves, the derived values reflected the true Km and Vm of isoprene synthase. Data presentation and the number of replicates as in Fig. 6.