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Time-of-drug-addition assay. BHK-21 cells were infected with FMDV at an MOI of 0.1 and then exposed to apigenin (20 µg/mL) at specific time points. −1 h: pre-infection 1 h. 0 h: during-infection. +1 h: post-infection 1 h. Apigenin exhibited a high inhibition rate at the post-infection stage. (A) Plaque reduction assay of apigenin; (B) FMDV viral titers. These experiments were performed three times, and the representative results are shown.
