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. 2015 Apr 8;7(4):1823–1831. doi: 10.3390/v7041823

Figure 2.

Figure 2

Analysis of Bag3 in VF formation (A) Levels of BAG3 silencing among five clones obtained from shBAG3 transduction in Vero cells. BAG3 clone number 3 (BAG3(3)) was the selected candidate for further experiments. Graphics in the lower panel show mean ± SD of WB quantification. (B) BAG3 expression in shBAG3(3) Vero cells and control (Scr) (upper panel); with proteasome inhibitor MG132 treatment (5 mM) for 16 h (middle panel) or infected with ASFV (moi = 1 pfu/cell). Capsid viral protein p72 (red) was used to detect VFs (lower panel). Asterisks denote statistically significant differences (*** = p < 0.001; ns = non significant). Bar = 10 μm.