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. 2015 Apr 20;7(4):2057–2073. doi: 10.3390/v7042057

Figure 2.

Figure 2

Western blot assay to detect viral protein production. (Left) HEK 293T cells were mock-infected or infected with either HSV-1 alone (MOI = 0.1) or HSV-1 treated with SP, A-beta, SEM amyloids, or SEVI for 24 h. Whole cell lysates were prepared for Western blot using antibodies against ICP27, ICP4, ICP8, gD, and tubulin (as loading control). (Right) The same as that in the left, but using HSV-2 instead of HSV-1; (Bottom) By comparing the density of the ICP27 bands of HSV-1 or HSV-2 with that of the virus alone (with all intensities normalized to the tubulin control), the fold-increases of ICP27 levels for HSV-1 (left) and for HSV-2 (right) were calculated (Quantity One software, version 4.5.0, Bio-Rad Laboratories, Richmond, CA, USA). Normalized ICP27 levels are shown below the corresponding Western blots.