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. 2015 Apr 22;8:15. doi: 10.1186/s13072-015-0006-8

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© Molden et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Histone H2B variant levels in myoblast cells compared to myotube cells. (A) Proliferating myoblast (prolif) and differentiated myotube cells (diff) were cultured in media containing heavy arginine, and samples were collected over the course of 7 days to monitor new histone synthesis. Heavy (new) over light (old) levels for histone variants in proliferating or differentiated cells were plotted over time for two biological replicates. (B) Relative abundances of histone H2B variants in myotubes (diff) versus (myoblasts). The abundance of each variant was summed and divided by the abundance of all of the variants. Mean ± SE, n = 3.