Table 1.
Sample | Raw reads | Post quality control | Total (paired and unpaired) reads for assembly in CLC | Total (paired) reads for trinity assembly | Contigs in CLC assembly | Contigs in trinity assembly |
---|---|---|---|---|---|---|
FL | 33,220,038 | 33,220,038 | 13,723,211 | 7,698,642 | 35,219 (33%) | 643 (67%) |
LC | 41,774,018 | 41,774,018 | 8,384,447 | 6,663,140 | ||
LSI | 51,696,682 | 51,696,682 | 11,101,071 | 8,835,358 |
Reads were mapped to Silva 111 database prior to assembly in CLC Workbench and Trinity to remove rRNA reads, but only paired reads were used for Trinity. The percentage value shown with the number of contiguous sequences (contigs) in each assembly is the percent of reads that mapped back to the assembly.