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. 2015 Apr 28;9:161. doi: 10.3389/fncel.2015.00161

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Copyright © 2015 Ferreira, Yousuf, Dalton and Sheets.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identifying double-labeled CP/CA neurons in IL. (A) Schematic depicting the concurrent injection of spectrally distinct retrograde fluorescent tracers into the BLA and PAG. (B) Confocal images at 10x and 25x magnification of retrogradely labeled CP (green) and CA (red) neurons in the IL cortex in a 50 μm coronal slice. Epifluorescent images at 60x magnification showing fluorescently labeled (C) CA neurons, (D) CP neurons, and the corresponding (E) overlay and (F) bright-field image. Arrows in each image identify the double-labeled CP/CA neuron. Rostral-caudal: R↔C; lateral-medial: L↔M.

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