Figure 7. Evaluating therapeutic impact of Ketorolac salt against DDX3 induced oral cancer by Immunohistochemistry and Immunoblot analysis.
Eight animals per group were used for this experiment. Following autopsy, the tongues were removed, fixed and embedded in paraffin. a) Photomicrograph of representative sections H&E staining and immunostained with DDX3 obtained at10X and 40X b) Tongue-tumor lysate proteins (20 μg/lane) were run in 10% SDS-polyacrylamide gel, electrophoresed and subsequently transferred to PVDF membrane. Immunoblots were probed with antibody for DDX3, Bcl-2, Mcl-1 and β- actin (loading control). C11-8 represent the control treated C2 and C3 1-8 represent 20 mg/kg abd 30 mg/kg Ketorolac treated groups respectively.