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. Author manuscript; available in PMC: 2015 Apr 28.
Published in final edited form as: Int J Hematol. 2014 Feb 22;99(4):393–406. doi: 10.1007/s12185-014-1534-x

Fig. 2.

Fig. 2

Exogenous Nov enhances LTR activity via the β3 integrin in the presence of TPO. a Forty CD150+CD34−KSL cells isolated from wild type (Wt) mice (Ly5.1) were cultured for 5 days with or without rmNov in the presence of TPO or SCF. The cultured cells were then transplanted into lethally irradiated mice (Ly5.2) along with 2 × 105 whole bone marrow (BM) competitor cells (Ly5.2). Twenty weeks after transplantation, the percentage of donor cells (Ly5.1) in the peripheral blood was determined, b Forty CD150+CD34−KSL cells derived from β3 integrin Y747A knock-in mutant mice (Ly5.2) were cultured with or without rmNov in the presence of TPO and subsequently transplanted along with 2 × 105 BM competitor cells (Ly5.1) into lethally irradiated recipient mice (Ly5.1) as described above. The plots represent the percentage of donor (Ly5.1 or Ly5.2)-derived cells in the peripheral blood of individual mice 20 weeks after transplantation. The bars depict the mean values (**P < 0.01). Recipient mice with donor cell chimerism <1.0 % for any lineage were not considered to be reconstituted (negative mice)