Effect of intra-NAc shell infusions of THIP on 10% ethanol (10E) intake. A) Squares depict injector tip locations for mice (n = 9) used in the drinking studies for nucleus accumbens (NAc) shell infusions with THIP. B) A sample photomicrograph of a mouse that received a bilateral NAc shell infusion of THIP. C) Effect of NAc shell THIP on total 2-hour 10E intake, D) average bout frequency, and E) 10E licks split into 20-minute bins. F) Horizontal locomotor activity (cm) following a NAc shell infusion of 0 or 500 ng THIP, subtracting out cm moved on a baseline day. The inset depicts total 2-hour activity. Values represent mean ± SEM for each dose. + p ≤ 0.10, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 relative to 0 ng.