(A) Mean (± SEM) number of NK3R immunoreactive cells/hemisection in POA, PVN, Rch, LHA and PMv of control (n=9) and prenatal T-treated (n=8) groups. There were no statistically significant differences between control and prenatal T-treated ewes in these areas. (B) Mean (± SEM) number of NK3R immunoreactive cells/hemisection in the middle and caudal ARC from control (n=9) and prenatal T-treated (n=8) groups. * indicates statistically significant differences within each subdivision compared to controls (P<0.05). (C–F) Representative images showing examples of NK3R-IR cells (arrows) in the ARC of control (C, mARC; D, cARC) and prenatal T-treated ewes (E; mARC; F, cARC). Scale bar = 50 µm.