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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Microvasc Res. 2014 Oct 2;97:55–64. doi: 10.1016/j.mvr.2014.09.008

Fig. 2.

Fig. 2

RUNX2-mediated EC differentiation is regulated through aldose reductase in HG. (A) The aldose reductase inhibitor ranirestat (100 nM) was added to ECs cultured on matrigel and tube formation was measured in starved cells (0 mM glucose) or cells treated with 5 mM or 25 mM glucose after 16 h. Bars represent mean area occupied by tubes; n = 4; *p < 0.01 (ANOVA). (B) ECs were starved for 16 h and treated for 4 h with the indicated doses of glucose. Nuclear extracts were examined for RUNX2 DNA binding after treatment of cells with 25 mM glucose and the AR-selective inhibitor ranirestat (0–500 nM). Relative band intensity normalized to total protein is shown for a representative assay (repeated three times). Ranirestat (100 nM) prevented inhibition of DNA binding in HG (NS, not significant, p > 0.05; lane 2 vs lane 7).