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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 May 10;74:53–63. doi: 10.1016/j.yjmcc.2014.04.024

Figure 5. Reduced connexin43 in αMHC-miR130a mice.

Figure 5

In panel (A), computational alignment of a potential conserved target site in the Cx43 3’UTR and miR-130a. In (B), representative timecourse of Cx43 protein loss after doxycycline removal in αMHC-miR130a hearts compared to controls (n=3, each group). After 2 weeks, we found a steady reduction of Cx43 with near complete loss by 10 weeks after doxycycline removal. In (C), quantitative PCR demonstrated a 52% reduction in Cx43 mRNA at 12 weeks after doxycycline removal (n=5, each group). Western analysis on ventricular lysates (panel D) at the same time point shows >90% reduction in Cx43 protein in αMHC-miR130a hearts compared to controls (n=10 in each group). In panel (E), western blot for both Cx43 and phosphorylated-Cx43 at 12 weeks after doxycycline removal demonstrates significant reduction in both forms of Cx43. * indicates p < 0.001.