Figure 5. Nlrp12−/− T cells are pathogenic.
. Purified CD4+CD45RBhi WT or Nlrp12−/− T cells were adoptively transferred into Rag1−/− mice. (A) Percent weight change over time. (B) Representative large intestine histology at 9 weeks post adoptive transfer of CD4+CD45RBhi T cells (original magnification ×20). (C) Combined colon histology scores. (D) Representative immunohistochemistry staining of T cells (anti-CD3), macrophages (anti-F4/80), neutrophils (anti-Gr-1), and cell death (TUNEL) in inflamed colon tissue (original magnification ×20). (E) Representative H&E ear sections (original magnification ×20). (F) Combined ear histology scores. Each point represents an individual mouse and the line represents the mean ± s.e.m. Data are representative of four independent experiments with 3-7 mice per genotype. *P < 0.05, **P < 0.01; Student's t-test. See also Figure S6.