. 2015 Mar 24;407(13):3817–3829. doi: 10.1007/s00216-015-8615-4

Table 2.

Comparison of mobile phase composition (φ _e) at moment of elution for peptides on the RP column with different ion-pairing agents. In the LC × LC-UV experiments, 0.05 % TFA was added to mobile phase A2, while for the LC × LC-MS/MS experiments 0.1 % FA was added to mobile phase A2

Peptide	Hydrophobicity index^a	² φ _e,TFA	² φ _e,FA
Bradykinin_1-5	7.5	0.05	0.02
Angiotensin	16.8	0.29	0.22
Neurotensin	27.2	0.38	0.31
ACTH_18-39	47.9	0.47	0.43
Bovine insulin	78.7	0.56	0.53

^aCalculation of the hydrophobicity index was based on [32]