Table 2. Differentially expressed copper-binding proteins identified by MS/MS.
| Spot no. a ) | Fold-ch. b ) 39/95 | NCBI Acc.no. c ) | Protein name | Mr/PI | SC d ) (%) | Score | PM e ) | MS/MS peptide sequence >95% C.I. (Indv. ion score) | |
|---|---|---|---|---|---|---|---|---|---|
| Theoretical | Observed | ||||||||
| Proteins involved in antioxidation and detoxification | |||||||||
| 5 | 4.41/3.88 a↑/b↑ | AAG46133 | Putative peroxidase | 35.4/7.55 | 42.1/5.16 | 5 | 130 | 1(1) | GLDAEDMVVLSGAHTVGR(130) |
| 22 | 2.4/3.2 b↑/a↑ | AAC04837 | Germin-like protein 6 | 24.5/5.92 | 26.6/6.54 | 28 | 388 | 6(5) | AAMLDTPR(42); YVNADHFFK(57); HSPVLVNGFACLDPK (98); IDYAPLGENPPHTHPR(120); GTIDWLQAQFWENNHY(71) |
| 28 | 2.5/2.1 a↑/a↑ | BAB92583 | Putative quinone-oxidoreductase(QR2) | 21.7/6.06 | 23.3/6.36 | 48 | 392 | 6(6) | VYVVYYSMYGHVAK(74); IWQVPETLHEEVLGK(83); WP TEMELEHAFHQGK(36); MFNMGEVQGGSPYGAGTFAAD GSR(109); MGAPPKPDVPTITPQELTEADGILFGFPTR(90) |
| 29 | 2.7/1.1 a↑/b- | Q7XUP7 | Methionine sulfoxide reductase A2-1 | 21.1/5.88 | 21.5/5.99 | 35 | 472 | 8(5) | HNPTTLNR(55); IVTEILPATR(62); TEVGYSQGHR(77); FYPAEEYHQR(65); DVCGGGTGHAEVVR(137) |
| 33 | 1.2/4.6 b↑/a↑ | AAA33917 | Copper/zinc superoxide dismutase | 15.3/5.71 | 17.7/6.29 | 15 | 175 | 2(2) | EHGAPEDETR(76); AVVVHADPDDLGK(99) |
| 16 | 3.8/1.8 a↑/b↑ | AAC64007 | Glutathione S-transferase II | 24.3/5.77 | 24.8/5.47 | 37 | 479 | 6(6) | VVEENLEK(65); VLEVYEAR(71); GEHKAPDHLAR(60); LYGSTLSWNVTR(91); NPFGQVPALQDGDLFLWESR(90); CVAVLEEAGAEYEIVPLDFSK(101) |
| 21 | 3.3/1.5 a↑/b↑ | AAC64007 | Glutathione S-transferase II | 24.3/5.77 | 26/6.3 | 27 | 358 | 5(5) | VVEENLEK(65); VLEVYEAR(75); GEHKAPDHLAR(50); LYGSTLSWNVTR(100); CVAVLEEAGAEYEIVPLDFSK(68) |
| Proteins involved in transcriptional regulation | |||||||||
| 8 | 2.32/1.72 a↑/b↑ | BAC65369 | CHP-rich zinc finger protein-like | 28.4/4.95 | 32.9/4.75 | 23 | 326 | 5(4) | DHDMKER(41); KMEDDFDAFTASK(75); IAIAVDLSDESA YAVR(117); LGSVSDYCVHHCVCPVVVVR(71); |
| 34 | new/none | BAC66711 | Putative cold shock protein-1 | 19.0/6.28 | 17.9/6.51 | 74 | 704 | 8(7) | GYGGGGGGYGGGDR(87); GYGGGGGYGGGGGGGS R(107); DCSQGGGGGGGYGGGGGGYR(136); SLNDGDVV EFSVGSGNDGR(138); GGGGGGGGGGCYNCGETGHI AR(102); AVDVTAPGGGALTGGSRPSGGGDR(45); GFGF ITPDDGGEDLFVHQSSLK(62) |
| Proteins involved in protein synthesis, modification, transport and degrdation | |||||||||
| 30 | 0.57/0.01 a↓/b↓ | ABA98689 | Putative eukaryotic translation initiation factor 5A-2 | 17.8/5.6 | 17.6/5.9 | 45 | 364 | 5(5) | TYPQQAGTIR(59); LPTDDNLLSQIK(99); CHFVAIDIFT AK(102); DLVVTVMSAMGEEQICALK(38); KLEDIVPSS HNCDVPHVNR(68) |
| 31 | 0.14/0.6 b↓/a↓ | ABF98987 | Putative eukaryotic translation initiation factor 5A-2 | 17.9/5.87 | 17.0/6.0 | 40 | 270 | 6(4) | NGHIVIK(37); TYPQQAGTIR(62); CHFVAIDIFNGK(79); DDLRLPSDEALLTQIK(43); |
| 32 | 0.57/0.02 a↓/b↓ | AAC67555 | Translation initiation factor 5A | 17.7/5.77 | 16.8/6.2 | 35 | 479 | 6(6) | TYPQQAGTIR(57); LPTDDSLLGQIK(84); CHFVAIDIFN GK(92); DDLRLPTDDSLLGQIK(54); LEDIVPSSHNCDVPHV NR(94); KLEDIVPSSHNCDVPHVNR(100) |
| 19 | 0.89/0.47 a-/b↓ | BAD26337 | Putative elongation factor EF-2 | 94.9/5.85 | 42.2/6.55 | 10 | 423 | 7(6) | GGGQVIPTAR(41); VIYASQLTAKPR(73); RVIYASQLTAKPR(56); ILSEEFGWDKDLAK(81); AYLPVIESFGFSSQLR(103); GHVFEEMQRPGTPLYNIK(46) |
| 4 | 1.57/1.52 a↑/a↑ | AAX85991 | Protein disulfide isomerase | 57.05/4.95 | 71.5/4.9 | 18 | 466 | 8(7) | NIQEYKGPR(40); GDAAVERPLVR(37); TADEIVDFIK K(64); VVVADNVHDFVFK(104); AHVEPDQIVSWLK(88); SDYDFGHTLHANHLPR(50); VVTFDKNPDNHPYLLK(56) |
| 35 | new/none | ABA99827 | Putative ubiquitin-conjugating enzyme spm2 | 16.8/6.42 | 16.5/6.52 | 46 | 292 | 6(4) | FSLLSNWR(39); EYTMEAILTQLKK(81); LFCDKDYPDRPPTVK(53); SWTGTIIGPHNTVHEGR(59) |
| 15 | 1.7/1.1 a↑/b- | BAA94966 | Epsilon-COP 1 | 31.7/5.23 | 27.5/5.18 | 32 | 551 | 6(6) | AVSAEDNFER(68); LSHPDHVLVK(48); EAYLIFQDFAE K(94); EWLSDSAVGSNPVLR(119); LIAGIIFMHEQDYTEA LK(112); NLFYLGAYQAAINNSDVPGLDADAAAER(109) |
| 10 | 3.23/2.23 a↑/b↑ | AAX11351 | Cathepsin B-like cysteine protease | 40.4/6.25 | 30.9/5.11 | 23 | 550 | 8(8) | HFSVNAYR(66); GWGDDGYFK(62); KHFSVNAYR(61); HITGGMMGGHAVK(78); PGCEPAYPTPVCEK(90); GTNEC GIEEDVVAGMPSTK(99); GVVTDECDPYFDQVGCK(93) |
| 1 | 2.21/2.34 a↑/a↑ | P25776 | Oryzain alpha chain; Flags: Precursor | 51.3/5.14 | 43.1/4.2 | 15 | 563 | 6(6) | SWGESGYVR(61); CGIAVEPSYPLK(71); AFQLYSSGIFTGK (102); CGIAVEPSYPLKK(70); AVANQPVSVAIEAGGR(99); CGTALDHGVAAVGYGTENGK(159) |
| 2 | 2.28/2.07 a↑/a↑ | P25776 | Oryzain alpha chain; Flags: Precursor | 51.3/5.14 | 42.1/4.24 | 8 | 222 | 3(3) | SWGESGYVR(42); AFQLYSSGIFTGK(88); AVANQPVSVAIEAGGR(93) |
| Pathogenesis-related protein | |||||||||
| 23 | 1.8/none a↑/ | BAC56823 | Putative pathogenesis- related protein | 18.2/4.76 | 19.6/4.25 | 20 | 220 | 3(3) | STTSIGCAR(37); ADYVYSSNTCTR(72); GALLDCGH YTQVVWR(111) |
| 26 | new/none | BAC56830 | putative pathogenesis-related protein | 19.4/4.37 | 11.7/4 | 41 | 216 | 4(3) | AGDCALIHSGSWEK(94); RVEGVGEVVWDDAVAA YAENYAAER(37); VDCDNGGVFITCNYNPAGNFQG ERPFER(54) |
| 25 | 7.5/2.3 a↑/b↑ | ABA99548 | Pathogenesis-related protein Bet v I family protein | 17.2/4.96 | 16.9/4.99 | 63 | 711 | 7(7) | AVAVSVER(55); APAFVSDER(94); VCLDVHSLPK(69) IVVCDSATHVLK(76); SHSTETKLEATGDATCVAK (158); LTVEYELEDGASLSPEQEK(149); VCAGFIDA VEVEGNGGPGTIHIMK(109) |
| 24 | 17.3/none a↑/ | AAF85972 | Pathogenesis-related protein PR-10a | 16.9/4.95 | 14.2/4.81 | 18 | 165 | 2(2) | VAVCDAASHVLK(55); APACVSDEHAVAVSAER(109) |
| Proteins involved in amino acid synthesis | |||||||||
| 6 | 2.35/5.81 b↑/a↑ | P14656 | Glutamine synthetase shoot isozyme | 39.4/5.51 | 40.8/5.32 | 13 | 407 | 5(5) | DIVDSHYK(60); NDGGYEIIK(74); EHISAYGEGNE R(70); HKEHISAYGEGNER(104); HETADINTFSWG VANR(99); |
| 11 | 0.34/none a↓/ | P14656 | Glutamine synthetase shoot isozyme | 39.4/5.51 | 31.5/5.27 | 13 | 250 | 5(5) | DIVDSHYK(67); NDGGYEIIK(72); EHISAYGEGN ER(91); HKEHISAYGEGNER(105); IIAEYIWIGGSG MDLR(49) |
| 12 | 0.13/none a↓/ | AAN05339 | Putative glutamine synthetase root isozyme | 38.8/5.73 | 33/5.36 | 17 | 456 | 6(5) | DIVDAHYK(63); GPITDVSQLPK(81); EHIAAYGEGNE R(86); HKEHIAAYGEGNER(103); IIAEYIWVGGSGID LR(109) |
| Protein involved in cell wall synthesis | |||||||||
| 14 | 2.3/1.1 a↑/b- | BAA81774 | Putative caffeoyl-CoA O-methyltransferase 1 | 27.9/5.11 | 27.5/5.13 | 38 | 570 | 8(6) | YVLDTTVLPR(59); YHEQLLQLVR(74); ESYEIGRPFL EK(51); GLEKLDELLAEEAAAGR(112); IDVCQLAIAD GITICR(119); EAAFDFAFVDADKPNYVK(108) |
| Protein involved in signal molecular | |||||||||
| 3 | 2.20/0.67 a↑/b- | BAA88900 | Calcium-binding protein | 48.07/4.52 | 40.3/4.56 | 18 | 469 | 7(6) | KVHTIFTK(56); NDKNHLIK(49); TLVLQFSVK(43); HEQKLDCGGGYVK(118); QSGSIYEHWDILPPK(89); FYAISAEYPEFSNKDK(91) |
| Proteins induced by salt stress | |||||||||
| 7 | 5.32/5.22 a↑/a↑ | AAB53810 | Salt gene product | 15.2/5.0 | 31.8/4.67 | 45 | 517 | 5(5) | LLGVTIYSSDAIR(90); SGTLIDAIGIYVHP(106); KLL GVTIYSSDAIR(110); EFSIPLQDSGHVVGFFGR(84); EISGTHGPVYDLADIVTYLK(126); |
| 27 | new/none | AAB53810 | Salt gene product | 15.2/5 | 9.2/5.04 | 51 | 506 | 6(5) | LLGVTIYSSDAIR(102); SGTLIDAIGIYVHP(108); KLL GVTIYSSDAIR(93); EFSIPLQDSGHVVGFFGR(92); EISGTHGPVYDLADIVTYLK(103) |
| 9 | 5.61/5.17 a↑/a↑ | A2WPN7 | Salt stress-induced protein | 15.2/5 | 33.1/5 | 66 | 656 | 6(6) | LLGVTIYSSDAIR(118); SGTLIDAIGIYVHP(108); KLL GVTIYSSDAIR(113); EFSIPLQDSGHVVGFFGR(118); EISGTHGPVYDLADIVTYLK(121); SIAFNYIGVDGQ EYAIGPWGGGEGTSTEIK(79) |
| The other proteins | |||||||||
| 18 | 2.9/2.1 a↑/b↑ | BAC83197 | Putative glyoxylate induced protein | 34.2/5.98 | 31.1/6.43 | 30 | 515 | 7(7) | DVNRDPLI(42); QPLLETPGEVFELR(102); HSVISDE VTTLVIFER(132); YTTIEGYHPDLIVGSTDK(44); A TNPTLAPAHLQDLPGFTR(68); RYTTIEGYHPDLIVG STDK(36); EVHYNQHGLLLLEGQGIYR(90) |
| 20 | 2.8/3.3 b↑/a↑ | NP_001060247 | Protein containing dimeric alpha-beta barrel domain | 26.5/7.11 | 25.5/6.01 | 16 | 373 | 4(4) | VSFGENFSPAR(82); LKEGVEAHQLAEK(89); SPAA EALGPTHVLHSR(117); LRSPAAEALGPTHVLHSR(85) |
| 13 | 1.1/0.21 a-/b↓ | AAO72629 | Adenosine kinase-like protein | 40.6/5.57 | 37/5.45 | 35 | 780 | 8(8) | KPENWALVEK(79); HLPMYDELASK(66); AGCYAAN VIIQR(82); GNVEYIAGGATQNSIR(155); VRGWETE NVEEIALK(76); VLPFVDYIFGNETEAR(115); IAVIT QGADPVVVAEDGQVK(128); NAQAAGVTAHYYEDE AAPTGTCAVCVVGGER(79) |
Note: Differentially expressed copper-binding proteins identified by MS/MS.;
a Spot no., numbering corresponds to the 2-DE gel;
b Fold-ch., Fold-changes, the fold-changes of each spot was calculated by %Vol in treated samples/%Vol in control samples;
↑, up-regulated;
↓, down-regulated;
-, no change;
c Acc. no., accession number in NCBI database;
d SC, sequence coverage by MS/MS;
e PM, number of peptides matched;
The a and b of letter about fold-changes indicate a statistically significant difference (p≤0.05) between B1139 and B1195 by Duncan’s test.