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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: Curr Biol. 2015 Mar 5;25(6):811–816. doi: 10.1016/j.cub.2015.01.053

Figure 3. Cyclin B3 Promotes the Metaphase-Anaphase Transition.

Figure 3

(A) The metaphase extension observed in Cyclin B3 RNAi-treated embryos was SAC independent, as a similar delay in metaphase was observed in SAC-deficient mad2p embryos (unpaired t test, control versus Cyclin B3 RNAi in mad2p embryos, p < 0.0001). Error bars represent the SD.

(B and C) Colchicine treatment in mad2p embryos with or without Cyclin B3 knockdown. Chromosomes were visualized by an H2AvD-GFP transgene. Mitotic exit was delayed in Cyclin B3 RNAi-treated embryos (chromosome decondensation at 07:44 in control versus 12:46 in Cyclin B3 RNAi-treated embryos). Scale bar, 5 mμ.

(D) Duration of mitotic phase in the Cyclin B3 knockdown embryos was significantly longer than that in either control or the other cyclin RNAi-treated embryos (unpaired t test, p < 0.0001). Error bars represent the SD.

See also Figure S3 and Movie S3.