TABLE 1.
Antiserum | Dilution | Immunogen | Source |
---|---|---|---|
Mouse anti-Brn3 | 1:50 | aa. 397–410 of human BRN3B* | Santa Cruz sc-6026 |
Mouse anti-calbindin | 1:500 | Purified bovine kidney calbindin-D-28 | Sigma C9848 |
Mouse anti-calretinin | 1:100 | Recombinant rat calretinin | Chemicon MAB1568 |
Sheep anti-Chx10 | 1:300 | Recombinant N-terminal aa. 1–131 of human CHX10 conjugated to GST | Exalpha Anti-Chx10 |
Mouse anti-glutamine synthetase | 1:200 | aa. 1–373 of sheep glutamine synthetase | BD Transduction Labs 610517 |
Mouse anti-p27Kip1 | 1:100 | Recombinant mouse p27Kip1 | BD Transduction Labs 610241 |
Mouse anti-Pax6 | 1:20 | Recombinant chicken Pax6 | DSHB Pax6 |
Mouse anti-rhodopsin | 1:400 | Rat retinal membranes | Sigma O4886 |
Goat anti-Sox2 | 1:100 | Recombinant C-terminus aa. 277–293 of human SOX2* | Santa Cruz sc-17320 |
Rabbit anti-Sox9 | 1:200 | Synthetic peptide VPSIPQTHSPQHWEQPVYTQLTRP from human SOX9 | Chemicon AB5535 |
Mouse anti-Syntaxin-1 | 1:200 | Synaptosomal plasma fraction of rat hippocampus | Sigma S0664 |
Based on matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) and liquid chromatography (LC) mass spectrometry performed in this study.