Fig. 2.

MiHA-specific CD8⁺ T cell priming and expansion from the naive repertoire is augmented by PD-L silenced DCs. Naïve (N, CD45RA⁺CCR7⁺) and effector memory (EM, CD45RA⁻CCR7⁻) CD8⁺ T cells were sorted and cultured for two consecutive weeks with HA-1 peptide-loaded PD-L silenced or control DCs (relative expression: PD-L1 7 %, PD-L2 12 %). The percentage (a) and cumulative numbers (b) of HA-1-specific CD8⁺ T cells obtained after stimulation with PD-L silenced or control DCs