Fig. 4.

Effect of blocking of NCRs on lysis of RMS cell lines by IL-15-activated NK cells. Combined data for the lysis of the RMS cell lines by IL-15-activated NK cells (5–7 donors, E:T ratio 10:1) in the presence of blocking antibodies to NKp30, NKp44 and NKp46 alone (solid bars) or together with the combined blocking antibodies to DNAM-1 and NKG2D (hatched bars). Data are depicted as percentage of the specific lysis obtained in the absence of a blocking antibody. Error bars represent SEM. Statistical analyses comparing combined blocking of DNAM-1 and NKG2D in the presence of blocking of the indicated NCR with combined blocking of DNAM-1 and NKG2D alone were performed using one-way ANOVA, followed by the Dunnett’s multiple comparisons test: p value <0.05 is indicated by *; <0.01 by **)