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Pluripotency evaluation and teratoma formation of hiPSCs. (a) Immunofluorescence staining with DAPI counterstain demonstrating positive pluripotency markers NANOG, OCT4, SOX2, and TRA-1-60. (b) H&E stains of a representative hiPSC-derived teratoma confirm pluripotency of the hiPSCs with presence of all three germ layers, including ectoderm, mesoderm, and endoderm. (scale bar = 400 μm)
