Fig. 3.

Morphology and Phenotypes of hiPSC-derived hiPSC-MSC cells. (a) Changes in cell morphology during hiPSC differentiation: Day 0: Dome-shaped hiPSC colony; Day 1: Changing the mTeSR1^™/E8 medium to hMSC medium leads to differentiation and out-growth of the cells from the colonies; Day 5: After sub-culture to uncoated and untreated culture flasks, the pre-differentiated cells attach to the polystyrene flask and start to form an elongated morphology. Day 21: At passage 4, cells show a spindle shape morphology, similar to hMSCs. (scale bar left panel = 400 and right panel 200 μm). (b) Flow cytometry analysis of surface markers of hiPSCs and hiPSC-MSCs at passage 4 shows positive hMSC surface markers of CD105, CD73, and CD90, and lack of CD45, CD34, CD14 or CD11b, CD19 and HLA-DR surface molecules according to the International Society for Cell Therapy (ISCT) criteria