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. 2015 Jan 13;11(2):242–253. doi: 10.1007/s12015-014-9581-5

Table 1.

Reference and target gene primer sequences used in qPCR experiments

Gene name Sequence
(F: Forward / R: Reverse)
Catalogue number Reference
GAPDH*

F: 5′ CGCTCTCTGCTCCTCCTGTT 3′

R: 5′ CCATGGTGTCTGAGCGATGT 3′

Hs02758991_g1 NM_001256799
ACAN

F: 5′ AGGCAGCGTGATCCTTACC 3′

R: 5′ GGCCTCTCCAGTCTCATTCTC 3′

Hs00153936_m1 NM_001135
SOX9

F: 5′ GTACCCGCACTTGCACAAC 3′

R: 5′ TCTCGCTCTCGTTCAGAAGTC 3′

Hs01001343_g1 NM_000346
COL2A1

F: 5′ CGTCCAGATGACCTTCCTACG 3′

R: 5′ TGAGCAGGGCCTTCTTGAG 3′

Hs00264051_m1 NM_001844
COL1A2

F: 5′ CAGGAAACAGCTATGACC 3′

R: 5′ CTACTCTCAGCCCAGGAGGTCCTG 3′

Hs01028969_m1 NM_000089
COL9A1

F: 5′ TGTAAAACGACGGCCAGT 3′

R: 5′ CAGGAAACAGCTATGACC 3′

Hs00932129_m1 NM_001851
COL10A1

F: 5′ GGCAGAGGAAGCTTCAGAAA 3′

R: 5′ AAGGGTATTTGTGGCAGCATA 3′

Hs00166657_m1 NM_000493
COL11A1

F: 5′ TGTAAAACGACGGCCAGT 3′

R: 5′ CAGGAAACAGCTATGACC 3′

Hs01097664_m1 NM_001190709