Fig. 8.

Effect of A/T-rich UP element on promoter recognition.

A. Effect of UP element on mutations at the −10 region of σ³² promoter. Activity of each mutant groE promoter with/without UP element are shown as a percentage of measured β-galactosidase activity of each wt promoter with/without UP element driven by wt σ³². Grey bars, P_groE-long (with UP element); clear bars, P_groE (without UP element).

B. Effect of UP element on promoter recognition mutant σ³². The bars show the activity ratios of mutant σ³² versus wt σ³². Grey bars, P_groE-long (with UP element); clear bars, P_groE (without UP element).

C. Suppression of promoter recognition mutant σ³² at grpE promoter by UP element from groE promoter (P_{groE_UP-grpE}). Assay strains are as described in Fig. 1A; promoter sequences are shown in Fig. 1B. Promoter mutations and variants, and σ³² derivatives are indicated below the x-axis. All values are averages of at least three independent experiments; error bars indicate one standard deviation.