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. 2015 Apr 28;10(4):e0124518. doi: 10.1371/journal.pone.0124518

Fig 4. Detection of BSMV in silenced leaves of Z. zerumbet by RT-PCR.

Fig 4

RNA was isolated from newly emerged leaves 30 days after infiltration with empty vector (control) and ZzPDS. Leaves were collected from the newly formed tillers as well. cDNA was prepared and RT-PCR was carried out using BSMV γ-specific primers. An amplicon of 309 bp representing BSMV γ transcript was obtained in all samples. M- 100bp ladder (NEB); Lane 1- Empty vector control; Lanes 2 & 3- Newly formed leaves of BSMV: PDS infiltrated plant 15dpi and 30dpi respectively; Lane-4- Leaf from new tiller 30dpi.