Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Ecol. 2015 Apr 20;24(10):2449–2462. doi: 10.1111/mec.13180

Figure 5.

Figure 5

Absorbance spectra of the regenerated New World warbler and Old World warbler Rh2 visual pigments. Main figure shows dark spectra and insets correspond to dark-light difference spectra for Rh2. The x-axis for insets has the same range as the main graph in all cases. Wavelengths of maximum absorbance (λmax) shown are the average of 3 different absorbance measurements. In addition to the ancestral Rh2 pigment present in most species, we expressed all the Rh2 visual pigments that had substitutions, except for D332E, which is not thought to affect spectral tuning (Yokoyama et al. 2008). Rh2 variant V1 corresponds to the one present in Setophaga pensylvanica, Rh2 V2 to that in S. fusca and Rh2 V3 is in S. castanea S. petechia and S. striata (See Fig. 2 and Table 3)