Table 1.
Sequences of primers used for quantitative (real-time) PCR
| No. | Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
| 1 | GAPDH | TGATGACATCAAGAAGGTGGTGAAG | TCCTTGGAGGCCATGTGGGCCAT |
| 2 | CyclinA | TAGACACCGGCAACTCAAG | TCTTCAGACTGGGAGAGGAGA |
| 3 | CyclinB1 | GAACAACTGCAGGCCAAAAT | CACTGGCACCAGCATAGG |
| 4 | CyclinE1 | GAAATGGCCAAAATCGACAG | TGTCAGGTGTGGGGATCA |
| 5 | CDK1 | TGGAGAAGGTACCTATGGAGTTG | AGGAACCCCTTCCTCTTCAC |
| 6 | CDK2 | AAAGCCAGAAACAAGTTGACG | GAGATCTCTCGGATGGCAGT |
| 7 | p21 | CTGGGGATGTCCGTCAGAAC | GCCATTAGCGCATCACAGT |
| 8 | p27 | AAACGTGCGAGTGTCTAACG | GTCCCGGGTTAACTCTTCGT |
| 9 | p53 | AGGTTGGCTCTGACTGTACC | GATTCTCTTCCTCTGTGCGC |
| 10 | p57 | CAGGAGCCTCTCGCTGAC | CCGGACAGCTTCTTGATCG |
| 11 | Caspase-3 | TGAGCCATGGTGAAGAAGGA | TCGGCCTCCACTGGTATTTT |
| 12 | Caspase-7 | ACCGGTCCTCGTTTGTACC | TTGTACTGATATGTAGGCACTCG |
| 13 | Caspase-9 | CTCTTGAGAGTTTGAGGGGAAA | ACTCACGGCAGAAGTTCACA |
GAPDH: glyceraldehydes-3-phosphate dehydrogenase.