Fig. 1.
Olanzapine inhibition of GlcPSe. Transport activity was measured in right-side-out (RSO) vesicles of JM1100 E. coli expressing GlcPSe. Final concentration of glucose in the assays was 30 μM. Unless otherwise specified, RSO vesicles were pre-incubated with different concentrations of olanzapine for 1 min before glucose uptake assay. Control is RSO vesicles of JM1100 E. coli cells transformed with empty pBAD vector. Olanzapine at 2 mM was pre-incubated for different time intervals (15 s, 30 s and 30 min). For the 2 mM washed sample, RSO vesicles were incubated with 2 mM olanzapine for 30 min, and then when olanzapine was removed by two washes: RSO vesicles were pelleted by centrifugation (3 min, 16,000g) and resuspensed in the same volume of buffer A. Error bars are standard deviations corresponding to 3 different measurements.