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. 2015 Apr 15;5:335–340. doi: 10.1016/j.fob.2015.04.006

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Glucose transport activity of GlcP_Se mutants. Glucose uptake of GlcP_Se mutants relative to wild-type, at 1 mM glucose, in E. coli JM1100 whole-cells assay. Error bars are standard deviations from 3 different experiments. Western blot of membranes extracted from cells expressing wild-type and mutants of GlcP_Se is shown below. Difference in band density in the Western film was within 30%: 100%, 109%, 117%, 116%, 128%, 104%, 123% and 118%, for wild-type, R71A, R129A, N136A, N191A, R367A, R369A and E362A GlcP_Se, respectively.