Figure 9.

Viral-mediated expression of iFGF14 in adult Fgf14^−/− Purkinje neurons. A, Schematic of the AAV transfer plasmid (see Materials and Methods) chicken β actin with CMV enhancer promoter (CAG). IRES, Internal ribosome entry site; ITR, inverted terminal repeat; pA, polyadenylation signal. B, C, Representative low-magnification (B) and high magnification (C) images of parasagital sections of adult Fgf14^−/− mouse cerebellum injected with AAV1 expressing GFP alone (top panels) or in combination with the AAV1 expressing iFGF14 (bottom panels). Scale bars: B, 25 μm; C, 5 μm. Anti-iFGF14 and anti-Ankyrin G immunostaining are shown in red and blue, respectively. Transduced Purkinje neurons were identified by GFP fluorescence.