Table 1.
Primers used for cloning, distribution, and quantification of the CbCCAPra
| Primer | Sequence (5′ to 3′) |
|---|---|
| cDNA library development | |
| Template switch (TS) | AAGCAGTGGTATCAACGCAGAGTACGCrGrGrG |
| TRsa(T)30 | CGCAGTCGGTACT30 |
| CapPCR | AAG CAG TGG TAT CAA CGC AGA GTA |
| Cloning primers | |
| Lu4Cap | CGACGTGGACTATCCATGAACGCAAAGCAGTGGTATCAACGCAGAGTA |
| Lu4TRsa | CGACGTGGACTATCCATGAACGCACGCAGTCGGTACT30 |
| NsLu4 | TCGAGCGGCCGCCCGGGCAGGTCGACGTGGACTATCCATGAACGCA |
| CbCCAPr.5.F | CCTACGTCTTGGTGGCTCTC |
| CbCCAPr.8.R | TAATCTTGGCCTTTGGGATG |
| CbCCAPr.4.R | AAGGGGAAGGTGATGACGGTCACT |
| CbCCAPr.7.F | ATCGATTTCCCGAAGTTGTG |
| Tissue distribution PCR primers | |
| CbCCAPr.5.F | CCTACGTCTTGGTGGCTCTC |
| CbCCAPr.8.R | TAATCTTGGCCTTTGGGATG |
| Cbβtub.1.F | TCTGTGCTGGATGTAGTCCG |
| Cbβtub.3.R | AGAGTGGCGTTGTATGGCTC |
| qRT-PCR primers | |
| CbCCAPr.3.F | GGTGGCTCTGACTGTCTTCCTCTT |
| CbCCAPr.4.R | AAGGGGAAGGTGATGACGGTCACT |
aPrimers used for development of cDNA libraries suitable for RACE, cloning of CbCCAPr, tissue distribution, and qRT-PCR measurements. βtub, Beta-tubulin; F, forward; R, reverse.