Fig. 4.

Inhibition of TgMAPKL-1 causes enlarged parasite cells. Parasites were treated with 250 nM 1NM-PP1 for 2 days. To observe the expression of HA-tagged TgMAPKL-1, an anti-HA antibody was used (shown in green). To observe the inner membrane complex underneath the parasite cell membrane, an anti-TgGAP45 antibody was used (shown in red). DNA was detected by use of DAPI (blue). Scale bars = 10 µm.