Table 1.

Primers used in the present study.

ID	Sequence (5'- > 3')
HA_F	GAAATGCATACCATGTACCCATACGATGT
HA_R	TGTGAATTCGCGTAATCTGGAACATCGT
5'UTR_F	CGGTATCGATAAGCTCAAGAAAAGCAACGAGAGAT
5'UTR_R	CGTGCTGATCAAGCTATGGTGGCGAAGAGTTGAG
R_GK_35	GACGAGGTAAATGTCCTCGAAATCTGGAGTCAG
F_GK_Ala	GACATTTACCTCGTCgcTGACCTGATGGACAC
F_GK_Tyr	GACATTTACCTCGTCTaTGACCTGATGGACAC
PCR_screen_1	TGAGTCAGAGCACGAGTGCCGGAGGCAGCCAAGCT
PCR_screen_2	CCGACAGAAGTCAAAAGGGAATGAGATGCCAGGTAT
PCR_screen_3	TATTTCTTCTGACCGCACGACCTTTCGCAGTTCAG
PCR_screen_4	AGCTGTTGCTGGCCATGCTGCAGCTGTTGTTGCCT

Boldface type shows the restriction enzyme sites. Underlined sequences are homologous sequences for the In-Fusion cloning system. Lower case italicized characters represent the mutated sequences.