Table 1.
Primers used in the present study.
| ID | Sequence (5'- > 3') |
|---|---|
| HA_F | GAAATGCATACCATGTACCCATACGATGT |
| HA_R | TGTGAATTCGCGTAATCTGGAACATCGT |
| 5'UTR_F | CGGTATCGATAAGCTCAAGAAAAGCAACGAGAGAT |
| 5'UTR_R | CGTGCTGATCAAGCTATGGTGGCGAAGAGTTGAG |
| R_GK_35 | GACGAGGTAAATGTCCTCGAAATCTGGAGTCAG |
| F_GK_Ala | GACATTTACCTCGTCgcTGACCTGATGGACAC |
| F_GK_Tyr | GACATTTACCTCGTCTaTGACCTGATGGACAC |
| PCR_screen_1 | TGAGTCAGAGCACGAGTGCCGGAGGCAGCCAAGCT |
| PCR_screen_2 | CCGACAGAAGTCAAAAGGGAATGAGATGCCAGGTAT |
| PCR_screen_3 | TATTTCTTCTGACCGCACGACCTTTCGCAGTTCAG |
| PCR_screen_4 | AGCTGTTGCTGGCCATGCTGCAGCTGTTGTTGCCT |
Boldface type shows the restriction enzyme sites. Underlined sequences are homologous sequences for the In-Fusion cloning system. Lower case italicized characters represent the mutated sequences.