Table 1.

Long-term BPQ treatments of N. caninum tachyzoites grown in human fibroblasts. HFF monolayers cultured in T25 tissue culture flasks were infected with N. caninum Nc-Liv tachyzoites. Treatments were carried out for 3, 6, or 9 days in the presence of various concentrations of BPQ. Culture post-treatment took place in the absence of compounds for a maximum time span of 35 days.

BPQ concentration (µM)	Duration of treatment (days)	Culture post-treatment (days)*	Parasitical effect (yes/no)
0	No treatment	3	No
0.1	20**	n.d.	No
0.5	3	3	No
	6	10	No
	9	35***	Yes
1.0	3	9	No
	6	35***	Yes
2.5	3	10	No
	6	35***	Yes

^*Numbers indicate the numbers of days of culture in the absence of BPQ following treatment until re-emergence of parasite proliferation was evident by the occurrence of plaques detected by light microscopy.

^**Parasite proliferation was observed after 20 days continuous culture in the presence of 0.1 µM BPQ.

^***No reappearence of Neospora after culture in the absence of BPQ, indicating parasiticidal effect.