FIG 3.
Antigen-specific CD8+ T cells induced by OVA-PIC (ε-PL) NPs. Mice (n = 3) were subcutaneously immunized twice with PBS, OVA (100 μg), OVA-PIC NPs (OVA-PIC) (1 mg of γ-PGA-Phe, 200 μg ε-PL, and 100 μg of OVA), or OVA plus alum (100 μg of OVA and 1 mg of alum). Spleen cells were isolated on day 14 after the final immunization. The cells were stained with an anti-CD8 MAb and the H-2Kb/OVA257–264 pentamer. The number in each panel indicates the percentage of CD8+ pentamer+ T cells. A representative result for one mouse in each group is shown.