TABLE 1.
Characterization of S. aureus isolates
| Isolatea | MLSTc result | CCd | spa typee | Allelic profile | spa repeat succession | Kreiswirth IDf | Virulence gene profile |
|---|---|---|---|---|---|---|---|
| 1B | ST1899 | 101 | t7760 | 3-1-14-15-11-212-3 | 04-13-12-17-20-17-12-17-17 | ZEGMDMGMM | fnbA, icaA, icaD |
| 2B | NAb | NA | t127 | NA | 07-23-21-16-34-33-13 | UJFKBPE | fnbA, icaA, icaD |
| 3B | ST1179 | 97 | t359 | 144-1-1-1-1-5-3 | 07-23-12-21-17-34-34-33-34 | UJGFMBBPB | fnbA, icaA, icaD |
| 4B | ST80 | 80 | t4152 | 1-3-1-14-11-51-10 | 07-23-12-02-12-34-34-33-34 | UJGAGBBPB | fnbA, icaA, icaD, pvl, mecA |
| 5B | ST1 | 1 | t127 | 1-1-1-1-1-1-1 | 07-23-21-16-34-33-13 | UJFKBPE | sea, seh, cna, fnbA, icaA, icaD, pvl |
| 6B | ST1 | 1 | t127 | 1-1-1-1-1-1-1 | 07-23-21-16-34-33-13 | UJFKBPE | sea, seb, seg, seh, sei, cna, fnbA, icaA, icaD |
| 1W | ST30 | 30 | t021 | 2-2-2-2-6-3-2 | 15-12-16-02-16-02-25-17-24 | WGKAKAOMQ | sei, cna, fnbA, icaA, icaD, pvl |
| 2W | ST239 | 239 | t037 | 2-3-1-1-4-4-3 | 15-12-16-02-25-17-24 | WGKAOMQ | sea, cna, fnbA, icaA, icaD, mecA |
| 3W | ST1290 | Singletons | t131 | 1-4-1-1-11-1-3 | 07-23-12-34-33-34 | UJGBPB | sea, fnbA, icaA, icaD, pvl |
| 4W | ST1 | 1 | t127 | 1-1-1-1-1-1-1 | 07-23-21-16-34-33-13 | UJFKBPE | sea, seh, cna, fnbA, icaA, icaD, pvl |
| 5W | ST239 | 239 | t037 | 2-3-1-1-4-4-3 | 15-12-16-02-25-17-24 | WGKAOMQ | sea, fnbA, icaA, icaD, mecA |
| 6W | ST1 | 1 | t127 | 1-1-1-1-1-1-1 | 07-23-21-16-34-33-13 | UJFKBPE | sea, sec, seh, cna, fnbA, icaA, icaD |
| 1H | ST1963 | 101 | t714 | 69-1-14-1-11-19-3 | 04-13-21-12-17 | ZEFGM | seb, sec, seh, fnbA, icaA, icaD |
| 2H | ST8 | 8 | t7758 | 3-3-1-1-4-4-3 | 11-19-21-17-21-34-23-25 | YHFMFBJO | sea, seh, fnbA, icaA, icaD, tssT |
| 3H | ST1964 | 121 | t159 | 3-5-6-2-7-14-5 | 14-44-13-12-17-17-23-18-17 | I2Z2EGMMJH2 M | seb, seg, sei, cna, fnbA, icaA, icaD |
| 4H | ST8 | 8 | t7759 | 3-3-1-1-4-4-3 | 11-10-34-33-25 | YC2BPO | seb, seh, fnbA, icaA, icaD |
| 5H | NAb | NA | t7463 | NA | 259-25-17-17-16-16-16-17-16-16-16-16 | OMMKKKMKKKK | sea, seg, seh, sei, fnbA, icaA, icaD |
| 6H | ST30 | 30 | t318 | 2-2-2-2-6-3-2 | 15-12-16-16-02-16-02-25-17-24 | WGKKAKAOMQ | sea, seg, seh, sei, cna, fnbA, icaA, icaD |
Cultured S. aureus isolates from 1B-6B patients were characterized as bacteremia strains, S. aureus isolates from 1W-6W patients were grouped as SSTI strains, and S. aureus isolates harvested from healthy 1H-6H participants were recognized as colonizer strains.
NA, not available. Seven sequences of internal fragments of housekeeping genes in the 2B S. aureus isolate were totally different from the alleles in the database; the aroE gene of the 5H isolate was not detected in this study.
MLST is a nucleotide sequence-based characterization of isolates that uses the sequences of internal fragments of seven housekeeping genes that are translated into a specific ST for each isolate.
CCs are defined as clusters of closely related STs with up to a single difference in the allelic profile.
spa type, Staphylococcus protein A gene typing is defined by polymorphic direct repeat regions in the spa gene. The repeats are assigned a numerical code, and the order of specific repeats is translated into a specific spa type for each isolate by the Ridom system (17).
Kreiswirth ID (18) is another nomenclature-based method of spa typing that assigns letters (A to Z, A2, B2, and more) to each repeat sequence rather than a numerical code. Subsequently, the combination of all of the letters is translated into a spa type motif.