FIG 5.

Reactivity of C. pecorum-specific individual cow and calf sera with C. pecorum peptides. (A) Heat map of reactivity of 17 cow and 19 calf sera with six C. pecorum E58 peptides. These bovine sera were obtained from animals in two herds with endemic C. pecorum infections. The cows had experienced multiple episodes of natural infection with multiple C. pecorum strains (72), while calves were sampled between 11 and 15 weeks of age, after serum colostrum antibodies had disappeared and calves had experienced a first episode of C. pecorum infection (20). Shading intensity is proportional to peptide reactivity with each serum (mean of two experiments, interassay CV = 7.5%), with a cutoff of 10,000 RLU/s. (B) Combined percent seropositivity of these single and multiple C. pecorum E58 peptides. Shaded areas indicate peptides not included in the combined reactivity of the evaluated peptides.