TABLE 2.
Highly reactive Chlamydia species-specific peptides
| Peptide | Sequence (5′–3′) | ELISA signal ([RLU/s] × 10−3)a | No. of sequence variants/total sequencesb | Peptides/species detectionc | ARR cross-reactivityd |
|---|---|---|---|---|---|
| Cab_S26/3_OmpA_153-176 | NLVGLIGVKGSSIAADQLPNVGIT | 365 | 2/39 | 1 | I |
| Cab_S26/3_PmpD_1060-1089 | KIESPTSNVYYSAHESVKQPENKTLADINS | 185 | 2/7 | 1 | II |
| Cab_S26/3_IncA_324-353 | STAVTEHADIPRDPNRDPRGGRGGQSSPSV | 109 | 2/5 | 1 | |
| Cps_02DC15_OmpA_158-181 | LVGLIGFSAASSISTDLPTQLPNV | 106 | 30/288 | 8* | III |
| Cps_05DC15_OmpA_250-265 | ASSNFPLPITAGTTEA | 78 | 17/204 | 4* | IV |
| Cps_02DC15_OmpA_333-348 | STTALPNNSGKDVLSD | 299 | 20/209 | 5* | III |
| Cps_02DC15_IncA_321-360 | SLTSTTETADQGDLRDPSGDRYGGWGAQSSYRLSPSVTMS | 328 | 7/77 | 4* | V |
| Cps_02DC15_CT618_105-134 | YEVDSATGSFKIVTKNIQKPNGEVEIVSSR | 249 | 3/41 | 2 | |
| Cps_02DC15_CT618_189-228 | CGAVDDVISIVSTLRSTDFDPSYEDLVQRRVTLREKFFSL | 343 | 3/41 | 2 | |
| Cca_GPIC_OmpA_159-168 | VTGTDLQGQY | 117 | 1/5 | 1 | |
| Cca_GPIC_IncA_316-355 | LIGVMVQDGAESSTVEEASQDDSAQPQDENQSDAGEHKDS | 321 | 1/4 | 1 | |
| Cca_GPIC_CT618_134-163 | EVDAQTGNFVLQTKTVQLEDGTQRVVPSRV | 383 | 1/3 | 1 | |
| Cca_GPIC_CT618_220-249 | ADDAVDIVSLVRSPTGDLSTEELSERRQTL | 180 | 1/3 | 1 | |
| Cfe_Fe/C_OmpA_160-175 | IGLAGTDFANQRPNVE | 46 | 1/10 | 1 | |
| Cfe_Fe/C_PmpD_1055-1084 | PNVKSVEKIESPSAKSYYSNYEIEKNPIEK | 52 | 1/6 | 1 | |
| Cfe_Fe/C_CT618_108-137 | DSASGNFKIGVKSVKNENGETVLVPCRILK | 528 | 1/3 | 1 | |
| Cpe_E58_OmpA_090-105 | TSPNNAADSSTTAERA | 48 | 24/203 | 8* | |
| Cpe_E58_OmpA_161-176 | ISGSSLEGKYPNANIS | 63 | 27/209 | 10* | |
| Cpe_E58_OmpA_323-338 | LGQATTVDGTNKFADS | 198 | 27/215 | 11* | |
| Cpe_E58_IncA_281-300 | AAPAAPAAPAAPAAPA | 110 | 14/126 | 9* | |
| Cpe_E58_IncA_311-326 | PAPENNDNNNDDNAAS | 513 | 8/89 | 5 | |
| Cpe_E58_CT529_209-248 | IIRERRAYQRCLERLNQKEVGQEESGSAQEVQAMRSSYVK | 154 | 2/15 | 1 | |
| Cpe_E58_CT442_151-190 | DGSNQIFVDSNRDIRRPGSGGSGGVSASGALEQVANIVMN | 94 | 3/19 | 3 | |
| Cpn_CWL029_PmpD_0147-0187 | EKISSDTKENRKDLETEDPSKKSGLKEVSSDLPKSPETAV | 80 | 1/16 | 1 | |
| Cpn_CWL029_PmpD_1131-1170 | NKEETLVSAGVQINMSSPTPNKDKAVDTPVLADIISITVD | 116 | 1/16 | 1 | |
| Cpn_CWL029_IncA_331-370 | QKAESEFIACVRDRTFGRRETPPPTTPVVEGDESQEEDEG | 340 | 2/13 | 2 | |
| Cpn_CWL029_CT618_201-216 | PETISDPENRNKPSAE | 215 | 1/13 | 1 | |
| Cmu_Nigg_PmpD_724-739 | KVETADINSDKQEAEE | 20 | 1/6 | 1 | |
| Cmu_Nigg_PmpD_1038-1053 | EIGDLEDSVNSEKTPS | 50 | 1/6 | 1 | |
| Cmu_Nigg_CT618_190-205 | TKDKCCDSTQGNSFME | 41 | 1/5 | 1 | |
| Csu_99DC3_OmpA_166-181 | FGLTTTSVAAQDLPNV | 6 | 25/55 | 22* | |
| Csu_99DC3_OmpA_317-332 | TISGKGQDAQTLQDTM | 20 | 20/40 | 12* | |
| Csu_99DC3_IncA_259-293 | LANIKEALIKPSRPPLPKNGFPRTMPPCPPRQTPP | 76 | 1/1 | 1 | |
| Csu_99DC3_CT529_207-236 | EERCNRILCGQADEVLGINNTMCEQFVRQR | 13 | 2/2 | 2 | |
| Ctr_D/UW-3_OmpA_082-105 | FQMGAKPTTDTGNSAAPSTLTARE | 166 | 28/601 | 11* | |
| Ctr_D/UW-3_OmpA_159-174 | FGDNENQKTVKAESVP | 20 | 22/741 | 10* | |
| Ctr_D/UW-3_OmpA_313-328 | IFDTTTLNPTIAGAGD | 535 | 11/738 | 3 | VI |
| Ctr_D/UW-3_OmpA_324-339 | AGAGDVKTGAEGQLGD | 73 | 18/725 | 6* | |
| Ctr_D/UW-3_OmpA_306-345 | QPKSATAIFDTTTLNPTIAGAGDVKTGAEGQLGDTMQIVS | 587 | 18/725 | 8 | VI |
| Ctr_D/UW-3_PmpD_536-565 | ARAPQALPTQEEFPLFSKKEGRPLSSGYSG | 3 | 3/201 | 1 | |
| Ctr_D/UW-3_PmpD_1036-1065 | SGTPVQQGHAISKPEAEIESSSEPEGAHSL | 6 | 5/202 | 3 | |
| Ctr_D/UW-3_CT529_200-239 | SAERADCEARCARIAREESLLEVPGEENACEKKVAGEKAK | 27 | 4/182 | 2 | |
| Ctr_D/UW-3_IncG_108-147 | RPSDQQESGGRLSEESASPQASPTSSTFGLESALRSIGDS | 40 | 4/183 | 2 |
Background-corrected average signals of six repeats with homologous sera at high stringency are shown. The signal for all peptides was highly significantly above background (P < 10−5; one-tailed Student t test). The method has an interassay CV of 11.2% and an intra-assay CV of 8.5%. All peptides were nonreactive above background with nonhomologous mouse sera against the eight remaining chlamydial species.
Sequence variants among all GenBank sequence accession numbers for the respective Chlamydia peptide sequences.
That is, the number of variant peptides within the respective Chlamydia spp. required to provide Chlamydia species-specific antibody binding to ≥95% of all GenBank sequence accession numbers at a ≤2 amino acid mismatch tolerance. *, peptides from this ARR show serovar-specific reactivity.
Potential for cross-reactivity of the antibody reactive peptide region (ARR) with other Chlamydia species/strains: I, Cps strains VS225 and GR9; II, Cps GR9 and possibly other Cps strains; III, Cab strains with Cps strains VS225 and GR9; IV, Cab strains with Cps strain VS225; V, ARR absent in Cps strain VS225, no reactivity; VI, central residues shared among all chlamydial species. Homologs from other species show unpredictable patterns of cross-reactivity.