TABLE 3.
Peptides with cross-reactivity among Chlamydia species
| Peptide | Sequence (5′–3′) | ELISA signal with Chlamydia monospecific antisera ([RLU/s] × 10−3)a |
No. of sequence variants/total sequences of reactive speciesb | No. of peptides/species detectionc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cab | Cps | Cca | Cfe | Cpe | Cpn | Cmu | Csu | Ctr | ||||
| Cab_S26/3_OmpA_089-104 | PTGTAAANYKTPTDRP | 23 | 0 | 0 | 0 | 0 | 22 | 0 | 0 | 0 | Cab (2/49), Cpn (2/36) | 1, 1 |
| Cab_S26/3_OmpA_223-262 | NVVSSPAQFVVHKPRGYKGTAFPLPLTAGTDQATDTKSAT | 0 | 0 | 0 | 0 | 0 | 39 | 1 | 0 | 1 | Cpn (3/37)* | 1 |
| Cab_S26/3_OmpA_309-324 | AVLNLTTWNPTLLGEA | 5 | 74 | 6 | 0 | 6 | 61 | 0 | 0 | 113 | Cab (1/38), Cps (14/132), Cpn (2/39) | 1, 4, 1, 3 |
| Cab_S26/3_OmpA_302-341 | AQPKLAAAVLNLTTWNPTLLGEATALDTSNKFADFLQIAS | 17 | 89 | 15 | 0 | 6 | 117 | 0 | 0 | 89 | Cab (2/38), Cps (20/209), Cca (1/5), Cpn (2/39) | 1, 7, 1, 1, 2 |
| Cab_S26/3_OmpA_366-389 | KWSITGEARLINERAAHMNAQFRF | 0 | 321 | 0 | 0 | 0 | 0 | 35 | 74 | 0 | Cps (4/287), Csu (1/33), Cmu (1/13) | 1 |
| Cab_S26/3_PmpD_1074-1113 | ESVKQPENKTLADINSIGIDLASFVSSDDETPVPPQIIVP | 136 | 269 | 40 | 134 | 0 | 0 | 0 | 0 | 0 | Cab (1/8), Cps (3/76), Cca (1/3), Cfe (1/6) | 1, 1, 1, 1 |
| Cca_GPIC_PmpD_571-610 | ITFSYNKGTILPFPKVAASSEGESAPEAPKESSPVDLGVR | 0 | 3 | 191 | 0 | 1 | 1 | 0 | 0 | 0 | Cca (1/3)* | 1 |
| Cfe_Fe/C_PmpD_569-598 | FSYNSGKFLPLPMPSAEVSEENSSQNAPVE | 0 | 0 | 182 | 0 | 0 | 0 | 0 | 0 | 0 | Cca (1/3)* | 1 |
| Cfe_Fe/C_PmpD_1083-1112 | EKTLADISSIGVDLASFVTNDDGSSPLPPQ | 116 | 214 | 151 | 260 | 0 | 1 | 1 | 0 | 0 | Cab (1/8), Cps (3/76), Cca (1/3), Cfe (1/6) | 1, 1, 1, 1 |
| Cpn_CWL029_PmpD_654-693 | EKSLNACSHGDHYPPKTVEEEVPPSLLEEHPVVSSTDIRG | 0 | 0 | 0 | 2 | 0 | 115 | 0 | 0 | 0 | Cpn (1/16)* | 1 |
| Cpe_E58_OmpA_313-328 | PIFNLTTWNPTLLGQA | 4 | 11 | 4 | 0 | 5 | 40 | 0 | 0 | 87 | Cpn (2/39), Ctr (11/738) | 4 |
| Csu_99DC3_OmpA_345-384 | RKSCGLAVGTTIVDADKYAVTVETRLIDERAAHVNAQFRF | 0 | 267 | 0 | 0 | 0 | 0 | 36 | 87 | 0 | Cps (1/287), Csu (1/33), Cmu (1/13) | 2, 1, 1 |
| Csu_99DC3_PmpD_535-564 | NARAPQAVPTRDPEEVFSLSAESLNGCSGG | 0 | 0 | 6 | 0 | 8 | 0 | 0 | 16 | 0 | Csu (2/2)* | 2 |
| Ctr_D/UW-3_OmpA_041-056 | EGFGGDPCDPCATWCD | 0 | 27 | 0 | 16 | 1 | 0 | 6 | 0 | 0 | Cps (4/284), Cfe (1/13) | 1, 1 |
| Ctr_D/UW-3_OmpA_104-119 | RENPAYGRHMQDAEMF | 0 | 0 | 0 | 0 | 0 | 119 | 2 | 0 | 197 | Cpn (1/36), Ctr (4/741) | 1, 2 |
| Ctr_D/UW-3_OmpA_080-119 | KEFQMGAKPTTDTGNSAAPSTLTARENPAYGRHMQDAEMF | 0 | 0 | 0 | 0 | 0 | 294 | 9 | 0 | 376 | Cpn (2/36), Ctr (24/763) | 1, 2, 16, 9 |
| Ctr_D/UW-3_OmpA_152-181 | SFNLVGLFGDNENQKTVKAESVPNMSFDQS | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 97 | Ctr (28/601) | 12 |
| Ctr_D/UW-3_OmpA_233-248 | EFTINKPKGYVGKEFP | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 41 | 100 | Csu (25/98), Ctr (8/750) | 10, 3 |
| Ctr_D/UW-3_OmpA_245-260 | KEFPLDLTAGTDAATG | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 105 | Ctr (9/750)* | 4 |
| Ctr_D/UW-3_OmpA_226-265 | NVLCNAAEFTINKPKGYVGKEFPLDLTAGTDAATGTKDAS | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 24 | 295 | Csu (28/98), Ctr (12/750) | 12, 4 |
a
That is, the average reactivity of three repeats of each peptide in a high-stringency ELISA with background subtracted (P < 10−3; one-tailed Student t test).
b
Sequence variants among all GenBank peptide sequence accession numbers for the reactive chlamydial species. *, despite experimental reactivity with only a single species, potential for cross-reactivity between species exists because of extensive shared peptide sequences.
c
Number of variant peptides within the respective Chlamydia spp. required to provide Chlamydia species-specific antibody binding to ≥95% of all GenBank sequence accession numbers at ≤2-amino-acid mismatch tolerance.