Figure 1.

Two dimensional-fluorescence difference gel electrophoresis (DIGE) gel of bovine serum proteins labeled with CyDyes. The protein extracted from the bovine serum of pregnant and non-pregnant as visualized by CyDye DIGE flour. First dimension was pH 3 to 10 NL IPG gel and second dimension was a range of 10 to 200 kDa in an 8% to 16% gradient gel. Comparison of proteomes of serum sample of pregnant and non-pregnancy Holstein dairy cattle day 21 after artificial insemination (A: Image overlay, B: mixture of pregnant and non-pregnancy bovine serum labeled with Cy2 for internal standard, C: non-pregnant serum labeled with Cy5 for Control, D: 21 days pregnant serum labeled with Cy3). NL, nonlinear. (Upper: fluorescence color images, lower: black and white-transformed images).